Comparative studies on molecular techniques for detecting Rickettsia tsutsugamushi in Leptotrombidium deliense and mice
نویسندگان
چکیده
In order to establish the most sensitive and specific method in the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense , mice and patients, a comparative study on DNA extraction, PCR, hybridization and PCR-hybridization was conducted. The results showed that the sensitivity of PCR-hybridization was 10 times higher than that of PCR and 1000 times higher than that of hybridization. Quick DNA extraction method could take the place of phenol-chloroform method to prepare DNA template when detecting the rickettsiae in mice and patients' blood by PCR. De-inhibitor quick DNA extraction method could improve the detection rate of rickettsiae from chigger mites. Key word s: Leptotrombidum deliense , Rickettsia tsutsugamushi , PCR, hybridization, PCR-hybridization
منابع مشابه
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